Regulatory

Part:BBa_K736000:Design

Designed by: Lethbridge iGEM High School Team   Group: iGEM12_Lethbridge_Canada   (2012-06-16)


Constitutive Promoter (J23100) with MLC (glucose-regulated) binding site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The EIIBC-gluc is responsible for proliferating the glucose signal to allow for transcription to occur.


Source

PCR was done using DNA primers designed for J23100, adding the MLC binding sequence to the 3' end. A modified VF2 was used for the 5' antisense.


References